Regulation of CHOP-10 in L-Glutamine-deprived SP2/0-Ag14 mouse hybridoma cells
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Regulation of CHOP-10 in L-Glutamine-deprived SP2/0-Ag14 mouse hybridoma cells

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Published by Laurentian University, School of Graduate Studies in Sudbury, Ont .
Written in English

Book details:

Edition Notes

Statementby Barry J. Deane.
SeriesCanadian theses = Thèses canadienes
The Physical Object
Paginationxv, 121 l. :
Number of Pages121
ID Numbers
Open LibraryOL22153757M
ISBN 100612856283

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Control of late apoptotic events by the p38 stress kinase in L-glutamine-deprived mouse hybridoma cells. GADD expression does not necessarily correlate with changes in culture behavior of hybridoma cells. Rapid induction of the intrinsic apoptotic pathway by L-glutamine starvation. Guerin PJ, Furtak T, Eng K, Gauthier ER. Oxidative stress is not required for the induction of apoptosis upon glutamine starvation of Sp2/0-Ag14 hybridoma cells. Eur J Cell Biol. ; – doi: /ted by: 6. In this paper, we report on the role which ammonium ions, a product of glutaminolysis, play on the survival of L-glutamine-deprived Sp2/0-Ag14 mouse hybridoma cells.   Growth inhibitive curve of cisplatin, etoposide and glutamine deprivation against HCC and BT cells. Cell viability of HCC cells (A) and BT cells (B) are measured by CCK-8 after cisplatin or etoposide treatment for 48 ine free medium pretreatment for 24 hours increases cisplatin- and etoposide-induced cell proliferation inhibition in BT cells (C, D) .

  The effects of glucose and l-glutamine on the growth and maintenance of recombinant Chinese hamster ovary (CHO) cells were investigated in batch ine was significant for cell growth although glutamine limitation was not observed above mmol l −ine was not critical to cell maintenance since cells grew a little and cell viability was as high as 90% for 40 h after the.   After a lag period, CHOP undergoes down-regulation releasing C/EBPβ from inhibitory constraint allowing transactivation of the C/EBPα and PPARγ genes, transcription factors required for terminal differentiation. Hybridomas Hybrid Cells B-Lymphocytes Cell Line T-Lymphocytes Chromosomes, Human, and X Lymphocytes Caco-2 Cells Cell Line, Tumor Tumor Cells, Cultured. Organisms 4. Mice, Inbred BALB C Herpesvirus 4, Human Flammulina Clostridium cellulolyticum. Diseases 3. Multiple Myeloma Epstein-Barr Virus Infections Plasmacytoma. Glutamine is the most abundant free α amino acid in most mammalian species. The plasma glutamine pool is turning over very rapidly since it plays important roles in the interorgan transport of carbon, nitrogen and energy [].Although present in the diet, most ingested glutamine is metabolized by the small intestinal mucosa and thus the large body pool of glutamine is synthesized de novo [].

Results A kinetic delay in the CHOP-/-UPR and identification of potential CHOP target genes. Transcriptional profiling was used to compare the complement of mRNAs in unstressed and ER-stressed wild-type and CHOP-/-primary mouse embryo fibroblasts (MEFs). As noted previously (Harding et al. ; Murray et al. ), exposure of cells to tunicamycin, a glycosylation inhibitor that perturbs. ATF4 and ATF2 are involved in the regulation of several amino acids regulated genes. In the upper panel we used wild type and ATF2–/– mouse embryonic fibroblast cells; in the lower panel we used HeLa cells transfected with ATF4 siRNA or control siRNA. Cells were incubated either in DMEM medium or in leucine-deficient medium for 8 h.   Cells were seeded at × 10 5 cells per well into 6-well plates or 5 × 10 4 cells per well into well plates and were allowed to attach for 7–12 h. ER, or the fusion of ER with MYC, were activated by adding 4-hydroxytamoxifen (OHT) (Sigma-Aldrich) to the media to a . A.L. McCall, in Encyclopedia of Stress (Second Edition), GLUT1 as a Stress-Related Protein. GLUT1, the ubiquitously expressed glucose transporter, which is constitutively expressed at low levels by most, if not all, cells, is regulated by cellular stress.A variety of cell stressors, including glucose starvation, mercaptoethanol, calcium ionophores, tunicamycin, and heat shock.